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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which results in chromosome instability, and thus may possibly add to tumorigenesis. This protein was uncovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential position in advertising and marketing mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two supplemental splice variants have been noted but specific nucleotide sequence details is just not but out there.
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Blocking peptides are peptides that bind specially on the goal antibody and block antibody binding. These peptide generally contains the epitope regarded with the antibody.
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This guideline outlines even more feasible optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guidebook outlines additional possible optimization of cellular and immunoassay parameters to be sure the very best final results are...
Antibodies sure to the blocking peptide no more bind to the epitope on the concentrate on protein. By evaluating the staining within the blocked antibody compared to the antibody alone, you can see which staining is certain.
Focus on Information Cyclin E belongs on the extremely conserved cyclin relatives, whose associates are characterized by a extraordinary periodicity in protein abundance in the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add into the temporal coordination of each and every mitotic event. Cyclin E types a fancy with and functions as a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S phase boundary and is also degraded as cells development by S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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